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Take Metro Line 2 (red) and get off at Acropoli station. Exit toward the Theatre of Dionysus / Acropolis. Walk about 2 minutes along Makrigianni Street to the bus stop.
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Some urban buses pass nearby (e.g., line 550 or A2 along Vasilissis Amalias), although the metro is more direct and simpler, since Acropoli station leaves you just 2 minutes’ walk from the stop.
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From Plaka, it’s a 7–10 minute uphill walk. From Syntagma, go down Vasilissis Amalias Street then turn toward the Acropolis (~15 minutes on foot).